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nerve growth factor  (R&D Systems)


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    Structured Review

    R&D Systems nerve growth factor
    Nerve Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nerve+growth+factor/Recombinant+Rat+beta-NGF+Protein/pmc13120944-45-16-20
    Average 93 stars, based on 66 article reviews
    nerve growth factor - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: Regulatory T cells require peripheral CCL2-CCR2 signaling to facilitate the resolution of medication overuse headache-related behavioral sensitization
    Article Snippet: Cells were dissociated by triturating with fire-polished glass pipettes, resuspended in MEM-based culture medium containing 5% fetal bovine serum, 25 ng/ml nerve growth factor (R&D, Minneapolis, MN) and 10 ng/ml glial cell-derived neurotrophic factor (R&D), and seeded on Matrigel-coated coverslips.

    Article Title: Structure matters: commensal Phocaeicola vulgatus lipopolysaccharide induces attenuated microglial activation and preserves neuronal integrity
    Article Snippet: For neuronal differentiation, the medium was replaced with RPMI containing 1% horse serum and 50 ng/mL nerve growth factor (NGF; R&D Systems, #556-NG).

    Article Title: Heat But Not Mechanical Hypersensitivity Depends on Voltage-Gated Ca<sub>V</sub>2.2 Calcium Channel Activity in Peripheral Axon Terminals Innervating Skin
    Article Snippet: Cells were resuspended in Neurobasal media supplemented with B27, Glutamax, Pen/Strep, and Nerve Growth Factor (256-GF, 0.1mg/ml; R&D Systems) and plated into 96-well plates (catalog #89626, Ibidi) precoated with 2mL dots of poly-D-lysine (0.1mg/ml; catalog #P6407, Sigma) and laminin (2.5mg/ ml; catalog #23017015, ThermoFisher).

    Article Title: An Innovative "Tooth-On-Chip" Microfluidic Device Emulating the Structure and Physiology of the Dental Pulp Tissue.
    Article Snippet: Then, the tgg medium, consisting of the neurobasal medium, B27 supplement (GibcoThermoFisher), 2 mM L-Glutamine (Gibco-ThermoFisher, 17504044), 100 U mL−1 penicillin and 100 μg mL−1 streptomycin, 50 ng mL−1 nerve growth factor (R&D Systems, 256-GF) and 0.25 pM cytosine arabinoside (AraC, Sigma-Aldrich/Merck, C1768), was added.

    Article Title: Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks.
    Article Snippet: Primary rat cortical neurons (RCN) prepared from the cortex of day-18 rat embryos (A10840-01, Life Technologies, Gaithersburg, MD) were suspended in a culture medium (21103-049, Neurobasal Medium, Thermofisher-Life Science) supplemented with 10 mL of B-27 supplement (17504044, Thermofisher-Life Science), 1% antibiotic (15140-122, Life Technologies), 5 mL of GlutaMax-I (35050-061, Thermofisher), and 50 μL of the nerve growth factor (0.01 μg/mL, 556-NG, RD systems).

    Article Title: An Innovative “Tooth‐On‐Chip” Microfluidic Device Emulating the Structure and Physiology of the Dental Pulp Tissue
    Article Snippet: Then, the tgg medium, consisting of the neurobasal medium, B27 supplement (Gibco‐ThermoFisher), 2 mM L‐Glutamine (Gibco‐ThermoFisher, 17504044), 100 U mL −1 penicillin and 100 μg mL −1 streptomycin, 50 ng mL −1 nerve growth factor (R&D Systems, 256‐GF) and 0.25 pM cytosine arabinoside (AraC, Sigma‐Aldrich/Merck, C1768), was added.

    Modification:

    Article Title: Mechanisms of sympathoexcitation via P2Y 6 receptors
    Article Snippet: Immediately after decapitation the ganglia were removed, cut into two pieces and incubated in a mixture of collagenase (1.5 mg ml −1 , Sigma-Aldrich, Vienna, Austria) and dispase (3.0 mg ml −1 , Roche, Vienna, Austria) for 30 min at 37°C, followed by an incubation in trypsin (0.25%, Worthington, Lakewood, NJ, United States) for 15 min at 37°C. .. Ganglia were washed twice with calcium free Tyrode solution (in mM: NaCl 150, KCl 4, MgCl2: 2, Glucose 10, Hepes 10, pH 7.4 adjusted with NaOH) and mechanically dissociated via trituration in Dulbecco’s modified Eagle’s Medium (DMEM, Sigma-Aldrich, Vienna, Austria) containing 10 mg L −1 insulin (Sigma-Aldrich, Vienna, Austria), 50 μg L −1 nerve growth factor (R&D Systems Minneapolis MN, United States), 25.000 IU L −1 penicillin (Sigma-Aldrich, Vienna, Austria) and 25 mg L −1 streptomycin (Sigma-Aldrich, Vienna, Austria). .. For electrophysiological and calcium imaging experiments, 35,000–50,000 cells were seeded into 0.5 cm diameter glass rings either placed in rat tail collagen coated (Trevigen, Minneapolis MN, United States) 35 mm tissue culture dishes (ThermoFisher scientific, Waltham MA, United States) or collagen coated glass bottom dishes (Mattek, Ashland MA, United States).

    Cell Culture:

    Article Title: Chondroitinase ABC/galectin-3 fusion proteins with hyaluronan-based hydrogels stabilize enzyme and provide targeted enzyme activity for neural applications.
    Article Snippet: Objective.. Chondroitinase ABC (ChABC) has emerged as a promising therapeutic agent for central nervous system regeneration.. Despite multiple beneficial outcomes for regeneration, translation of this enzyme is challenged by poor pharmacokinetics, localization, and stability.



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